"We want to get along with Russia, but Russia is looking out and saying, 'Man, I wish she [Clinton] won'," he claimed at a rally in Duluth, Minnesota.
The president has been criticized internationally for ordering the separation of migrant children from their parents jailed after they crossed illegally the southern US border.
He signed an executive order on Wednesday canceling the practice.
Trump has been accused by Democrats of colluding with Russia during the 2016 election campaign.
He hit back at Clinton’s party, suggesting the Democrats wanted to "stay on immigration" to avoid being confronted in Congress on the "Russian scam."