The Iranian Foreign Ministry has summoned the Swiss charge d'affaires, who is representing US interests in the country, according to the official website.
Tehran has also issued a "strong protest against warmongering statements in violation of the United Nations Charter by US officials".
The statement comes amid escalating tensions in the region, as protestors stormed the American Embassy in Baghdad after the US military carried out several airstrikes against Shia militants in Iraq and Syria.
Washington accused the Islamic Republic of orchestrating the attack on the embassy, claiming that Tehran would be held fully responsible for any damage caused to the facility by the pro-Iranian protestors. The Islamic Republic denied the allegations, urging the US to abandon its "destructive policies" in the Middle East.
In the meantime, US Defence Secretary Mark Esper vowed to boost the American presence in the region, sending about 750 servicemen to the Middle East following the clashes in Baghdad.