Speaking to supporters in Beirut's southern suburbs on Saturday, Nasrallah said that Iran will not "sell its allies down the river during the nuclear talks" because "there was no bargaining" between Iran and the United States.
"Iran's relationship with its allies is based on ideological grounds and comes before political interests," Nasrallah said.
He recalled that Iran's Supreme Leader Ayatollah Ali Khamenei "reiterated Iran's position on the resistance movement and its allies," and Hezbollah occupies a special place among them.
"We say this loud and clear: we receive material and financial support from the Islamic republic, and we are proud of that fact," Nasrallah pointed out.
His remarks came a week after Khamenei warned that despite the nuclear deal, Iran would continue to pursue its policy towards the "arrogant" United States and support Tehran's friends in the region.
While blacklisted by the United States as a terrorist organization, Hezbollah also fights alongside President Assad's forces against militants in Syria.
Last month, the US Treasury slapped sanctions on three Lebanese men, who were allegedly part of a "key Hezbollah support network." According to Nasrallah, the sanction will not have a negative impact on his group.