In the letter, the European Commission warned the 28 EU ambassadors in Brussels that the deal will not be signed unless Washington changes its approach to the negotiations.
The document was sent from the office of European Agriculture Commissioner Phil Hogan and was agreed by several Commission departments. The office of EU Commission President Jean-Claude Juncker was aware of the email, Politico reported.
The document focuses in agriculture, one of the most contentious chapters in the TTIP talks.
"The EU has not seen substantial progress in areas of significant importance to EU agriculture, such as geographical indications, wine and non-tariff barriers," the email read.
"The US Administration does not yet seem to be in a place where it can reciprocate the EU’s efforts in TTIP and to start delivering on matters of EU interest," it added.
The document came in response to correspondence received last week by the EU Commission from US Ambassador to the EU, Anthony Gardner. He criticized Hogan for making a "series of misleading statements in the press."
Gardner added that the US position is that "EU tariffs are 2-3 times as high as US tariffs and EU non-tariff barriers have virtually eliminated many of our key exports," according to Politico.
However, an internal EU Commission briefing obtained by the website shows that Brussels has its own concerns over the possible removal of tariffs for US products.
If tariffs and some non-tariff barriers are fully removed by Brussels the US will receive substantial gains for its agriculture, the document read.
"In the applied scenarios the US is a clear winner in trade in agricultural products in TTIP," it read.
The TTIP deal has been controversial since it was initiated. Its backers, including US President Barack Obama, say that it would create the world’s largest free-trade zone. They also say it would reduce trade tariffs on products.
Recently, German Vice Chancellor Sigmar Gabriel questioned the need for further negotiations with the US on the TTIP. He also criticized Chancellor Angela Merkel for premature steps on the issue.
Thus, German Vice Chancellor Sigmar Gabriel has opposed German Chancellor Angela Merkel's "hasty" approach toward the deal.
"This was wrong that in the euphoria of Obama's visit to Germany, the Chancellor said we will be able to conclude negotiations by the end of this year and recently repeated this statement once again," the politician said in an interview with RND.
The negotiations on the TTIP have been underway since July 2013. In addition to the US and the EU, a number of countries are expected to join the initiative, including Canada, Mexico, Switzerland, Lichtenstein, Norway and Iceland.
Mikhail Belyaev, senior economist at the Institute of Stock Market and Management, said that the TTIP is an American economic invasion of Europe.
"The terms of the deal are not acceptable for a country trying to preserve its sovereignty. This is the kind of partnership that would justify a US economic invasion in Europe. It’s also about politics. American capital will invade in Europe, and access to the European market for US products will be much easier. This is economic aggression," Belyaev told Radio Sputnik.
According to the expert, the US is likely to achieve its goals because the EU lacks capabilities and the political will to oppose the deal.