He said that he ordered a Ukrainian state company that services the country's air traffic to strengthen control over any flights of Russian aircraft to Syria.
"The airspace for these [Russian] aircraft [flying to Syria] over the territory of Ukraine is closed; and the airspace over neutral waters is now controlled in a special way," Yatsenyuk said in Bratislava.
Moscow has yet to comment on the move made by Kiev. However, since 2014 Russia has not been using Ukrainian airspace for transit air operations, RIA Novosti reported.
Moreover, the US embassy appealed to Athens on Saturday with a request to prohibit the flights of Russian aircraft providing aid to Syria, however, Greece refused to do so, a source told RIA Novosti.
However, on Wednesday, Iran satisfied all of Moscow's requests and opened the country's airspace to Russian planes delivering humanitarian aid to the Middle Eastern country.