"The decision by the Canadian authorities on the further expansion of sanctions against official Russian figures and companies under the guise of the events in Ukraine look like a clumsy attempt to hamper the fulfilment of the agreements on settling the conflict reached in Minsk on February 12 with the active and constructive role of Russia," Lukashevich said in a statement published on the ministry's website.
Earlier on Wednesday, Canada imposed sanctions against 37 individuals and 17 companies from Russia and Ukraine.
The diplomat added that Canada's sanctions would not be left without a response from Moscow.
"Canada's stake on sanctions will obviously not go unanswered. We hope that Ottawa will consider the consequences of its actions, which are actually feeding the military conflict in Ukraine, and realize the unlikelihood of pressuring Russia with sanctions," Lukashevich said, adding that "defining the future direction of Russian-Canada relations depends entirely upon whether Canada behaves responsibly and demonstrates common sense."
Wednesday's blacklist comes on the heels of last week's decision by EU foreign ministers to impose asset freezes and travel bans against 19 individuals and nine entities in Russia and Ukraine, which came into force Monday.
Canada's previous sanctions against Moscow, announced in December 2014, restricted export of technology in the Russian oil and gas industry.
Over the past year, the United States, the European Union and allied Western nations introduced several rounds of anti-Russia sanctions targeting its banking, energy and military sectors, citing Moscow's alleged involvement in the Ukrainian crisis.
Russia remains adamant it is not involved in Ukraine's internal affairs.