"I think he should no longer be able to speak on behalf of the National Front," Marine Le Pen told Europe 1 radio, iTele TV channel and Le Monde newspaper in an interview.
"I have a feeling that he does not support that National Front continues to exist while he is no longer in charge. I regret this."
On May 1, Jean-Marie Le Pen reportedly walked onto the stage uninvited when his daughter was giving a Mayday address in Paris.
France will hold presidential elections in 2017. Marine Le Pen is expected to make a strong bid in the upcoming vote, as her party was elected second in France's departmental elections in March.