"Neither the vessels nor the cargo belongs to Iran. The cargo included gasoline that was sold by Iran to Venezuela. The US seized not the property of Iran, but the property of Venezuela," the minister said, as quoted by the IRIB broadcaster.
On Friday, the US announced that it had confiscated "a multimillion-dollar fuel shipment" by the Islamic Revolutionary Guard Corps from four Venezuela-bound tankers.
The US did not specify when "the largest-ever seizure of fuel shipments from Iran" had taken place.
President Hassan Rouhani earlier said that the US claims were meant to cover up the "shame and humiliation" from the defeat of its UN Security Council resolution on extending arms embargo on Iran.