US Congresswoman Nydia Velazquez said Congress needs to develop a legal framework for exchanging crypto-currencies and asked Zuckerberg whether he would be willing to help.
"My understanding is that Congress exercises significant oversight over the regulators through these committees, so that would seem to me like the appropriate way for that to happen", Zuckerberg replied.
"So that is a no," Velazquez responded before relinquishing the microphone.
Zuckerberg's apparent reluctance to work directly with Congress coincided with a torrent of criticism, especially from Democrats on the House of Representatives Financial Services Committee, amid a spate of legal woes faced by Facebook.
The United States recently fined Facebook $5 billion for failing to protect data from the platform's 2.2 billion users.
Facebook also faces fines of similar magnitude from the European Union for multiple violations of European Union privacy protections.
In addition, 47 US states and jurisdictions have joined the state of New York in investigating whether Facebook violated US anti-monopoly laws.
Committee Chair Maxine Waters set the tone of the hearing by devoting nearly all of her five-minutes for questions to a speech criticizing Facebook for running discriminatory housing advertisements, allowing foreigners to use the platform to meddle in US elections and a policy of not fact-checking political advertisements.
Other lawmakers pressed Zuckerberg on issues ranging from encryption to content promoting child sex abuse on Facebook.
Anger in Congress has reached a point where some lawmakers advocate breaking up Facebook into smaller companies - a strategy used by the United States in the early 20th century to weaken powerful industrial monopolies.