Four Russians - astronauts Oleg Artemyev, Sergei Ryazansky, Doctor Alexei Baranov and sports physiologist Alexei Shpakov - and two members of the European Space Agency, France's Cyrille Fournier, a civilian pilot, and mechanical engineer, Germany's Oliver Knickel, will take part in the experiment.
Initially, six Russians and four Europeans wanted to participate in the institute's project.
All participants will receive 15,500 euros ($20,000) each, Institute spokesman Mark Belakovsky said.
Institute Director Igor Ushakov said the 105-day trial is a continuation of the 14-day experiment of November 2007 and precedes the main event, a 520-day simulation flight due to start in late 2009.
The 520-day experiment will simulate all aspects of the journey including the 250-day one-way trip to the Red Planet, a 30-day stay on its surface, and the 240-day return flight.
During nearly two years of isolation, crew members will experience many of the conditions likely to be encountered by astronauts on a real space flight.
They will stick to a rigid daily regime of work, rest and exercise, and follow the diet of crews aboard the International Space Station (ISS).