Speaking at a UN Security Council session, Churkin specifically noted Washington's "extremely destructive" role in the Ukraine crisis.
"One can only hope that the US will not put a wrench in the works of the Normandy process," Churkin said.
He spoke almost two weeks after the Normandy format talks, which came amid new violence in eastern Ukraine's Donbas region, where government troops clashed with rebel militias.
During the February 12 talks, the leaders of Russia, Ukraine, France and Germany adopted a series of measures stipulating, in particular, the introduction of a cease-fire in the Donbas region as of February 15, the withdrawal of heavy weapons and the creation of a security zone.
The ceasefire regime in eastern Ukraine has generally been observed, according to the parties to the conflict and international monitors.