"The return of these two facilities to Russia while the Kremlin refuses to address its influence campaign against the United States would embolden President Vladimir Putin and invite a dangerous escalation in the Kremlin's destabilizing actions against democracies worldwide," Senators Jeanne Shaheen, Marco Rubio and Johnny Iakson warned in their letter to Trump on Thursday.
The senators wrote their letter ahead of a scheduled face-to-face meeting between Putin and Trump on Friday on the sidelines of the G20 Summit in Hamburg.
Senior Trump administration officials have said that the two leaders could discuss the return of the two diplomatic compounds in the US states of New York and Maryland.
But the three senators urged Trump to "remove the return of these facilities from any negotiation or consideration in your discussions with President Putin during your upcoming trip."
Former President Barack Obama shut down the two compounds in December last year, amid allegations that Russia interfered in the 2016 US presidential election.
Russia denies meddling in the vote, insisting that it does not interfere in the internal affairs of other countries.