"I officially declare that the rumour of the sunken submarine is not true to fact", Tolkachev has said to reporters on Thursday.
In question is the diesel museum submarine The Novosibirski Komsomolets, brought to Moscow last autumn.
Some Russian media recently reported that, after water was discharged from the Khimki water reservoir, the submarine went to the bottom.
Tolkachev said that the sunken sub rumour has been provoked by organisations interested in getting budget money for landscaping the reservoir bank at which the submarine stands.
According to the representative of the Moscow government in the Federation Council, the design of building a military machines museum on the Khimki reservoir is ready. The submarine, built in 1971, will become one its exhibits.
Now the submarine is on the premises of the refrigeration plant at the Khimki reservoir bank opposite to where the museum is going to be built.
"As soon as navigation opens, we will move the submarine to the other bank", Tolkachev said.