The Israeli Defense Forces (IDF) have deployed its defence systems and are "on high alert for an attack," after identifying what it said was "irregular activity of Iranian forces in Syria".
The IDF has also ordered local authorities in the Golan Heights, controlled by Israel, to "unlock and ready (bomb) shelters."
Following US President Donald Trump's Tuesday announcement that the US would be withdrawing from the 2015 Iranian nuclear deal, Israel also stated that it would be mobilizing some of its forces.
"We are drafting reservists in specific cases, as required," a military spokesperson told Reuters.
Earlier this week, reports circulated in the media that Iran was preparing response to alleged Israeli attacks on Iranian facilities in Syria in late April.
Relations between Tel Aviv and Tehran has been tense over the past time, with Israel viewing Iranian activities in Syria as a threat to national security. Israel has repeatedly claimed that Iran was allegedly building a permanent military base south of country's capital of Damascus.
February was marked by a major escalation in the conflict between Israel and Iran in Syria when the Israeli army said its helicopter intercepted an Iranian drone launched from Syria and that the Israeli Air Force (IAF) struck Iranian targets in Syria. The Israeli moves prompted fire from Syrian air defense systems, resulting in an Israeli F-16 fighter jet being hit. In the second wave of the attack, the Israeli forces struck 12 targets in Syria, including Syrian air defense batteries and Iranian military facilities, the country's Defense Forces (IDF) said.