The coalition strikes occurred near al-Tanf town, where US' and British special operations forces have been training Syrian rebel fighters near the border with Iraq and Jordan.
"The coalition struck pro-regime forces… after apparent Russian attempts to dissuade Syrian pro-regime movement south towards al-Tanf were unsuccessful, a coalition aircraft show of force and the firing of warning shots," the statement said.
The deconfliction memprandum on flights over Syria set up between the United States and Russian military remains in focre despite the US-led coalition's strike on Syrian government troops, the coalition added.
"The agreed upon de-confliction zone agreement remains in effect," the statement said.
The United States and Russia signed the bilateral memorandum of understanding in October 2015 to ensure their flight safety during combat missions over Syria.
On Monday, Al-Masdr News reported that Syrian Arab Army soldiers along and militias affiliated with the Iraqi Popular Mobilization Units are preparing to recapture the nearby Tanf Border crossing from Daesh.