"While we have today filed our appeal so that Londoners can continue using our app, we hope to continue having constructive discussions with Transport for London. As our new CEO has said, we are determined to make things right," Uber stated.
Citing "public safety and security implications," London's transport regulator deemed Uber "not fit and proper" to operate in the city last month.
It listed concerns about the company's approach to reporting serious criminal offenses, a lack of background checks on drivers and Uber's use of "Greyball" software that blocks access to the app for regulators and law enforcement.
Uber also set up a petition to persuade Transport for London and city mayor Sadiq Khan to reconsider their decision, which has been signed by 850,000 Londoners.