"No, it is totally not on the agenda. This is not even his wish, he said so himself," he replied to the BFMTV.
In a surprise announcement on Wednesday, Bayrou said he had "sacrificed" his bid for presidency to support independent candidate Macron, who agreed to an alliance with the veteran politician.
A recent Ifop survey found Macron would beat National Front party leader Marine Le Pen in the May run-off voting with 61 percent against 39 percent if they both make it to the second round. The first round of presidential election is scheduled for April 23.