The published financial statements say the conglomerate lost $49.7 billion in the first quarter. The holding's revenue in 2019 amounted to $254.6 billion.
"As efforts to contain the spread of the COVID-19 pandemic accelerated in the second half of March and continued through April, most of our businesses were negatively affected, with the effects to date ranging from relatively minor to severe", the company said.
Meanwhile, the company's operating profits were 6 percent higher than those of 2019, amounting to $5.87 billion, or about $3,388 per share.
Berkshire owns railroad Burlington Northern Santa Fe, insurer Geico and chocolate maker See's Candies.
The businesses were rocked in mid-April due to numerous restrictive measures and lockdowns, which halted many industries across the world.
According to the Johns Hopkins University count, the new coronavirus pandemic has infected more than 3,402,400 people and has claimed the lives of at least 242,000 worldwide. In the United Kingdom, the COVID-19 tally has surpassed 183,400, with over 28,200 fatalities, the university says.