Leaders of Protestant churches on Wednesday submitted a request to the Prosecutor General's Office asking for the channel's broadcasting license to be revoked.
The satellite channel broadcasts animated comedies including Futurama and South Park, and regularly issues a parental advisory that children under 14 should not watch the shows.
"Through the use of cartoons this channel is pumping, day and night, an ideology into the consciousness of minors of perversion and other vices, pitilessness and violence, promotion of homosexuality, religious hatred, and intolerance," a statement from the advisory council of the heads of the Russian protestant churches addressed to Prosecutor General Yury Chaika said.
The statement includes a request to ban several popular animated shows in Russia, and to revoke the channel's license.
The church leaders said Japanese animated series Battle Vixens promotes violence and contains elements of child pornography, Beavis and Butthead creates a culture of moral imbecility among Russian teenagers, Angry Kid advocates violence and vulgarity, and the American show South Park contains hidden propaganda of homosexuality and pedophilia as norms of sexual life.
In a similar case six years ago a Russian citizen, Igor Smykov, initiated a trial asking to take the world's best-known cartoon family, the Simpsons, off the airwaves in Russia. He claimed that the Simpsons show promoted drugs, violence, and homosexuality and had an unwholesome effect on his son, and demanded $10,000 in compensation from the channel Ren TV
The trial lasted three years and eventually the Moscow City Court turned down Smykov's appeal.